This prestained protein MW marker is designed for monitoring the progress of SDS-polyacrylamide gel. Please contact us at for specific academic pricing. Thermo Scientific PageRuler Plus Prestained Protein Ladder is a mixture of nine (9) blue-, orange- and green-stained proteins (10 to 250kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting. Additional data should be considered for a more accurate adjustment.Īpproximately 0.2~0.4 mg/ml of each protein in buffer (20 mM Trisphosphate pH 7.5 at 25☌), 2% SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15% (v/v) Glycerol.ĥ μl of BlueRAY Prestained Protein Ladder (PM006-0500) resolves 10 bands in 4-20% SDS-PAGE (Tris-glycine buffer) and after Western blotting to PVDF membrane. Note: The molecular weight of each protein (kDa) was measured against an unstained protein ladder in every electrophoresis condition. ![]() 2.5 μl per well for general Western transferring. Caution: Not intended for human or animal diagnostic or therapeutic uses.ģ μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. Note: The apparent molecular weight (kDa) of each protein has been determined by calibration against unstained protein standards supplemental data should be considered for more accurate adjustment in different electrophoresis conditions. Refer to the Iris9 Prestained Protein Ladder patterns in various electrophoresis conditions: Guide for Molecular Weight Estimation (kDa) Review other protein ladders in the unstained and prestained protein ladder guide. Recommended loading: 2 - 3 µl for western blots. Easy to identify: Includes green 25 kDa and red 75kDa reference bands. Ready-to-use: Supplied in a loading buffer for direct loading on gels. The ladder is supplied in gel loading buffer and is ready to use. Key product features: Broad range: 10-180 kDa. Nitrocellulose) and for approximate sizing of proteins. The Iris9 Prestained Protein Ladder is designed for monitoring protein separated during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon or Proteins are covalently coupled with a blue chromophore except for four reference bands (two green bands at 15 kDa & 60 kDa and two red bands at 30 kDa & 70 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). The Iris9 Prestained Protein Ladder is a three-color protein standard with 9 pre-stained proteins covering a wide range molecular weight from 15 to 180 kDa.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |